Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India

Authors

  • Ram Narayan Sharma Ram Division of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, India
  • Devinder Sharma Division of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, India
  • Biswajit Brahma Division of LPM, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, India
  • Pradeep Kumar Kumawat ivision of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, India
  • Sharad Kumar Meena Department of Entomology, Vasantrao Naik Marthawada Krishi Vidhyapeeth, Parbhani, Maharashtra, India

DOI:

https://doi.org/10.13102/sociobiology.v71i4.10394

Keywords:

A. Mellifera, incidence, IAPV, KBV, SBV, TSBV

Abstract

The present investigation was conducted from 2019 to 2020 to study the incidence pattern and molecular diagnosis of major honeybee (Apis mellifera L.) viral diseases in different areas of Jammu region of Jammu and Kashmir (UT). Using the RT-PCR technology, research was done to confirm that A. mellifera is infected by the Israeli acute paralysis virus (IAPV), the Thai sacbrood virus (TSBV), the Kashmir bee virus (KBV), and sacbrood virus (SBV). Particular primers for distinct viruses verified the existence of the virus in chosen samples from various areas, including Jammu, Samba, Ramban, and Udhampur. The samples taken from all four districts were found to contain illness. Clear and distinct bands with molecular sizes of roughly 110, 122, and 119 bp for IAPV, KBV, and SBV were formed by primer pairs of various viruses. The four districts’ samples contained no evidence of the Thai sacbrood virus. The incidence range of Israeli acute paralysis disease was 8.33 to 21.42 %. The incidence range for Kashmir bee disease was 5.71 to 28.33 %. The incidence range for sacbrood disease was 8.0–18.55%. In the apiary at SKUAST-J, Chatha, the incidence ranges for IAPV, KBV, and SBV diseases were 0 to 6.25, 0 to 4.16, and 0 to 9.43 %, respectively.

Downloads

Download data is not yet available.

References

Ai, H., Yan, X. & Han, R. (2012). Occurrence and prevalence of seven bee viruses in Apis mellifera and Apis cerana apiaries in China. Journal of Invertebrate Pathology, 109: 160-164.

Allen, M. & Ball, B.V. (1996). The incidence and world distribution of the honey bee viruses. Bee World, 77: 141-162.

Allen, M., Ball, B.V., White, R.F. & Antoniw, J.F. (1986). The detection of acute paralysis virus in Varroa jacobsoni by the use of a simple indirect ELISA. Journal of Apicultural Research, 25: 100-105.

Bailey, L. (1963). The habitat of bacterium Eurydice. Journal of General Microbiology, 31: 147-150.

Cox-Foster, D.L., Conlan, S., Holmes, E.C., Palacios, G., Evans, J.D., Moran, N.A., Quan, P.L., Briese, T., Hornig, M., Geiser, D.M., Martinson, V., vanEngelsdorp, D., Kalkstein, A.L., Drysdale, A., Hui, J., Zhai, J., Cui, L., Hutchison, S.K., Simons, J.F., Egholm, M., Pettis, J.S., Lipkin, W.I. (2007). A metagenomic survey of microbes in honey bee colony collapse disorder. Science, 318: 283-287.

De Miranda, J.R., Cordoni, G. & Budge, G. (2010). The acute bee paralysis virus-Kashmir bee virus-Israeli acute paralysis virus complex. Journal of Invertebrate Pathology, 103: 30-47.

Francis, R.M. & Kryger, P. (2012). Single assay detection of acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus. Journal of Apicultural Science, 56: 137-146.

Govan, V.A., Leat, N., Allsop, M. & Davison, S. (2000). Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses. Virology, 277: 457-463.

Grabensteiner, E., Ritter, W., Carter, M.J., Davison, S., Pechhacker, H., Kolodziejek, J., Boecking, O., Derakhshifar, I., Moosbeckhofer, R., Licek, E. & Nowotny, N. (2001). Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using reverse transcription-PCR. Clinical and Diagnostic Laboratory Immunology, 8: 93-104.

Martin, S.J., Highfield, A.C., Brettell, L., Villalobos, E.M., Budge, G.E., Powell, M., Nikaido, S. & Schroeder, D.C. (2012). Global honey bee viral landscape altered by a parasitic mite. Science, 336: 1304-1306.

Nielsen, S.L., Nicolaisen, M. & Kryger, P. (2008). Incidence of acute bee paralysis virus, black queen cell virus, chronic bee paralysis virus, deformed wing virus, Kashmir bee virus and sacbrood virus in honey bees (Apis mellifera) in Denmark. Apidologie, 39: 310-314.

Rana, B.S. & Rana, R. (2015). Detection of sacbrood virus and the incidence of sacbrood disease in Apis mellifera colonies in the North-Western Himalayas. Journal of Apicultural Research, 59: 144-149.

Rana, R., Rana, B.S. Kaushal, N., Kumar, D., Kaundal, P., Rana, K., Khan, M.A., Gwande, S.J. & Sharma, H.K. (2011). Identification of sacbrood virus disease in honeybee Apis mellifera L. by using ELISA and RT-PCR techniques. Indian Journal of Biotechnology, 10: 274-284.

Rustemoglu, M. & Sipahioglu, H.M. (2019). Occurrence and prevalence of six honey bee viruses in Hakkari (Turkey) and their genomic divergence. Munis Entomology and Zoology, 14: 574-583.

Todd, J.H., Miranda, J.R.D. & Ball, B.V. (2007). Incidence and molecular characterization of viruses found in dying New Zealand honey bee (Apis mellifera) colonies infested with Varroa destructor. Apidologie, 38: 354-367.

Downloads

Published

2024-11-25

How to Cite

Ram, R. N. S., Sharma, D., Brahma, B., Kumawat, P. K., & Meena, S. K. (2024). Nucleic Acid-based Identification of Major Honeybee (Apis mellifera L.) Viruses in the Jammu Region of India. Sociobiology, 71(4), e10394. https://doi.org/10.13102/sociobiology.v71i4.10394

Issue

Vol. 71 No. 4 (2024)

Section

Research Article - Bees

License

Copyright (c) 2024 Ram Narayan Sharma Ram, Devinder Sharma, Biswajit Brahma, Pradeep Kumar Kumawat, Sharad Kumar Meena

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Sociobiology is a diamond open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Authors who publish with this journal agree to the following terms:

 

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

 

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

 

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).